Temperature-Sensitive Mutants of Influenza Virus. II. Attenuation of ts Recombinants for Man
Identifieur interne : 000210 ( 1968/Analysis ); précédent : 000209; suivant : 000211Temperature-Sensitive Mutants of Influenza Virus. II. Attenuation of ts Recombinants for Man
Auteurs : Brian R. Murphy [États-Unis] ; Elias G. Chalhub [États-Unis] ; Sandra R. Nusinoff [États-Unis] ; Robert M. Chanock [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1972.
Abstract
Recombinant viruses were produced that contained the surface H3 antigen of influenza A/Hong Kong/1968 (H3N2) virus and a temperature-sensitive (ts) lesion derived from a ts mutant of influenza A/Great Lakes/1965 (H2N2) virus. Three recombinants, each bearing a genetically distinct ts lesion, were administered intranasally to volunteers who lacked serum neutralizing antibody for the H3 hemagglutinin. The ts recombinants exhibited a decreased virulence for man, but the level of attenuation varied. One of the recombinants, ts-l [E], appeared to possess the degree of attenuation desirable for a virus to be used in a live vaccine. The majority of men who were infected by the ts-l [E] recombinant did not develop symptoms. Those symptoms that did occur were mild. Infection with the ts-l [E]recombinant was extensive enough, however, to stimulate moderate levels of serum and nasal neutralizing antibodies and to induce complete resistance to influenzal disease produced by challenge with a virulent wild-type virus. The ts-l [E] recombinant was genetically stable in man and failed to spread from infected individuals to susceptible cohorts who were in close contact. The use of a ts lesion that imposes a defined and desirable degree of attenuation upon influenza A virus and the transfer of this lesion to new antigenic variants of the virus offer some hope for the rapid development of a live vaccine for containment of pandemic disease.
Url:
DOI: 10.1093/infdis/126.2.170
Affiliations:
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II. Attenuation of <hi rend="italic">ts</hi> Recombinants for Man</title><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Chalhub, Elias G" sort="Chalhub, Elias G" uniqKey="Chalhub E" first="Elias G." last="Chalhub">Elias G. 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Three recombinants, each bearing a genetically distinct ts lesion, were administered intranasally to volunteers who lacked serum neutralizing antibody for the H3 hemagglutinin. The ts recombinants exhibited a decreased virulence for man, but the level of attenuation varied. One of the recombinants, ts-l [E], appeared to possess the degree of attenuation desirable for a virus to be used in a live vaccine. The majority of men who were infected by the ts-l [E] recombinant did not develop symptoms. Those symptoms that did occur were mild. Infection with the ts-l [E]recombinant was extensive enough, however, to stimulate moderate levels of serum and nasal neutralizing antibodies and to induce complete resistance to influenzal disease produced by challenge with a virulent wild-type virus. The ts-l [E] recombinant was genetically stable in man and failed to spread from infected individuals to susceptible cohorts who were in close contact. The use of a ts lesion that imposes a defined and desirable degree of attenuation upon influenza A virus and the transfer of this lesion to new antigenic variants of the virus offer some hope for the rapid development of a live vaccine for containment of pandemic disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name></region><name sortKey="Chalhub, Elias G" sort="Chalhub, Elias G" uniqKey="Chalhub E" first="Elias G." last="Chalhub">Elias G. Chalhub</name><name sortKey="Chanock, Robert M" sort="Chanock, Robert M" uniqKey="Chanock R" first="Robert M." last="Chanock">Robert M. Chanock</name><name sortKey="Nusinoff, Sandra R" sort="Nusinoff, Sandra R" uniqKey="Nusinoff S" first="Sandra R." last="Nusinoff">Sandra R. Nusinoff</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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